Fig. 1
From: Macrophage hypoxia signaling regulates cardiac fibrosis via Oncostatin M
Cardiac infiltration of MΦ after TAC operation. a Data represent the cardiac infiltration of MΦ (CD11b+, F4/80+, Ly6G− mononuclear cells) after TAC operation. b Data represent the cardiac infiltration of Ly6Chi MΦ and Ly6Clo MΦ after TAC operation. *Ly6Chi MΦ. †Ly6Clo MΦ. The Kruskal–Wallis test was used for the statistical analysis. (Figure a, b; n = 5 at day 0, 3, 7, 14, and 28). c Hypoxic area was visualized in TAC operated mice (3 days after TAC) using LOX-1 (red). Vasculatures and nucleus were labeled with isolectin (green) and Hoechst 33342 (blue) (left). Scale bar = 50 μm. LOX-1 signal intensity was calculated in sham or TAC operated mouse (right). (ROI number 180, sham and TAC operated mouse, respectively). d MΦ after TAC operation were stained with Pimonidazole (Pimo). The population of Pimo positive cells in Ly6Chi MΦ (day 3, n = 5) and Ly6Clo MΦ (day 7, n = 5) are shown. e Cardiac infiltration of Ly6Chi MΦ and Ly6Clo MΦ in myeloid-specific HIF-1α conditional knockout mice (mHIF-1α CKO: HIF-1αflox/flox; LysM-cre+/−) were analyzed 3 or 7 days after TAC operation. Cre negative littermates were used as a control (cont). Day 3, cont (n = 6) and mHIF-1α CKO (n = 4). Day 7, cont (n = 3) and mHIF-1α CKO (n = 4). The Mann–Whitney U test was used to compare differences (c, d, e). Error bar represents the standard deviation. n.s. not statistically significant. * or †, p < 0.05
