Fig. 4
From: Macrophage hypoxia signaling regulates cardiac fibrosis via Oncostatin M
OSM gene expression is induced in hypoxia through a HIF-1α dependent manner. a Thioglycollate-elicited peritoneal macrophages (TEPMs) were isolated from hemtopoietic/endothelial-specific HIF-1α knockout mice (HIF-1α KO) or cre negative littermates as a control (cont). The TEPMs were exposed to hypoxia (1% O2), and the relative expression level of Osm mRNA was analyzed. The one-way ANOVA and Dunnett’s multiple comparisons test were used for the statistical analysis (F (2, 6) = 90.20). †p < 0.05 vs 0 h. The two-way ANOVA and Sidak’s multiple comparison test was performed for the statistical analysis (F (2, 12) = 94.66). *p < 0.05 vs cont. b HIF-1α binding to the promoter of Osm gene was studied by chromatin immunoprecipitation coupled to detection by quantitative PCR. Primer set 1 and 2 were designed to detect the promoter legion (−500 and −170 bp) of Pgk1 gene. Primer set 3 and 4 were designed to detect the hypoxia response element (HRE, −4 kb) of Osm gene. Data show the mean and the standard deviation (effort bar) of technical triplicates from a representative experiment. Quantitative PCR analysis were repeated at least three independent experiments. Two-tailed t-test with Welch’s correction was used for the statistical analysis (Primer 1 (t = 1.527, df = 3.863), Primer 2 (t = 93.69, df = 2.011), Primer 3 (t = 197.7, df = 3.202), Primer 4 (t = 75.41, df = 2.505)). n.s. not statistically significant. *p < 0.05
