Fig. 4

Liver-resident Kupffer cells mount IFN-β responses in a STING-dependent manner. IFN-β reporter (rep) (Ifnb^wt/Δβ-luc), AlbCre⁺IFN-β reporter (AlbCre^+/−Ifnb^floxβ-luc), CD11cCre⁺IFN-β reporter (ItgaxCre^+/−Ifnb^floxβ-luc), LysMCre⁺IFN-β reporter (Lyz2Cre^+/−Ifnb^floxβ-luc), and CD169Cre⁺IFN-β reporter (CD169Cre^+/−Ifnb^flox-βluc) mice were i.v. infected with 5 × 10⁵ pfu MCMV Δm157. a At the indicated time points luciferin was i.v. injected and luciferase activity was monitored by in vivo imaging. b Liver was marked as region of interest and luminescence intensity was quantified. c At the indicated time points luciferin was injected i.v. and luciferase activity was monitored by in vivo imaging. d Spleen was marked as region of interest and luminescence intensity was quantified. Data represent at least two independently performed experiments and one representative mouse out of at least four similar ones is shown. Error bars indicate mean ± s.e.m. (IFN-β reporter n ≥ 7, AlbCre⁺IFN-β reporter n ≥ 9, CD11cCre⁺IFN-β reporter n ≥ 5, LysMCre⁺IFN-β reporter n ≥ 7, CD169Cre⁺IFN-β reporter n = 4; *p ≤ 0.0424, **p ≤ 0.0061, ***p ≤ 0.0006; ns = not statistically significant; a two-tailed Mann–Whitney test was used to calculate p-values)