Fig. 5
STING is important for the control of early hepatic MCMV. C57BL/6, IFNAR KO (Ifnar−/−), MyTrMa KO (Myd88−/−Trif−/−Mavs−/−), STING KO (Tmem173−/−), and MyTrMaSt KO (Myd88−/−Trif−/−Mavs−/−Tmem173−/−) mice were i.v. infected with 5 × 105 pfu MCMV Δm157luc. a At the indicated time points luciferin was i.v. injected and the luciferase activity was monitored by in vivo imaging. One representative mouse out of six similar ones is shown. b Liver and c the cervical area were marked as region of interest and luminescence signals were quantified. Data are from at least two independently performed experiments. Error bars indicate mean ± s.e.m. (C57BL/6 n ≥ 7, IFNAR KO n = 6, MyTrMa KO n ≥ 6, STING KO n = 6, MyTrMaSt KO n = 6; *p ≤ 0.0350, **p ≤ 0.0047, ***p ≤ 0.0007; a two-tailed Mann-Whitney test was used to calculate p-values). C57BL/6, MyTrMa KO, STING KO, and MyTrMaSt KO mice were infected as indicated above with MCMV Δm157 and mice were perfused with PBS on 1, 3, and 5 dpi. d Liver and e spleen were removed, weighed and viral titers in pfu per gram organ were determined by a plaque assay. Data are from at least two independently performed experiments. Dashed line (DL = Detection Limit). Bars indicate mean (C57BL/6 n = 7, MyTrMa KO n ≥ 6, STING KO n ≥ 8, MyTrMaSt KO n = 6; *p ≤ 0.0381, **p ≤ 0.0072, ***p ≤ 0.0006; ns = not statistically significant; a two-tailed Mann–Whitney test was used to calculate p-values)
