Fig. 6
7D10-H retaining neutralizing activity after viral cell attachment. a Neutralization curves. 7D10-H IgG, 7D10-H Fab, 7D10 scFv were tested for neutralizing activities against pseudotyped MERS-CoV. VRC01 mAb was used as unrelated control. b Pre- and post-attachment neutralizing activities. 7D10-H IgG was tested for neutralizing activity against pseudotyped MERS-CoV before or after receptor binding. VRC01 mAb was used as unrelated control. c The effect of 7D10-H Fab on the conformational change of the MERS-CoV S trimer was probed by western blotting using an anti-MERS-CoV S2 polyclonal antibody. Refolding to the postfusion conformation was detected by the appearance of a proteinase-K resistant band. Trypsin was used at 5 μg mL−1 and proteinase K at 10 μg mL−1. Digestion experiments and western blots were performed in triplicates, and a representative result is shown for each of them. d A cartoon representation designed by us showing the neutralization mechanism by which 7D10 blocks MERS-CoV entry. On the one hand, some virus particles can not bind to DPP4 due to steric hindrance caused by 7D10 binding. On the other hand, 7D10 still recognizes the particles when the up receptor-binding domain (RBD) binds to DPP4, and may inhibit the prefusion to postfusion transition of the S2 subunit and the initiation of membrane fusion. Source data are provided as a Source Data file
