Fig. 1

Loss of E2F4 leads to defects in mouse ES cell growth. a Representative brightfield images (scale bar, 400 µm) and alkaline phosphatase (AP) staining (wells from a 6-well plate are shown) of wild-type (WT) and E2F4KO (KO) colonies one week after plating single cells (n > 10 assays per genotype). b Quantification of the size of AP⁺ colonies (unpaired t-test; n = 3 biological replicates per clone). c Comparison of the area of AP staining (undifferentiated cells) versus Giemsa staining (all cells) in an independent set of colonies (n = 3 biological replicates per clone) (no significant differences). d Size of WT and E2F4KO individual cells as estimated by forward scatter in flow cytometric analysis (unpaired t-test; n = 2 biological replicates per clone). e Total number of cells per 10 cm dish one week after plating at low density (unpaired t-test; n = 3 biological replicates per clone). Data shown as the mean and standard error of the mean