Fig. 5

Loss of E2F4 leads to defects in the growth of RB family TKO mouse ES cells. a Representative brightfield images (scale bar, 400 µm) and alkaline phosphatase (AP) staining (wells from a 6-well plate are shown) of RB family triple knockout (TKO) and RB family knockout, E2F4KO (QKO) colonies one week after plating single cells (n > 10 assays per genotype). b Quantification of the size of AP⁺ colonies (unpaired t-test; n = 2 biological replicates per clone). c Total number of cells per 10 cm dish one week after plating at low density (unpaired t-test; n = 2 biological replicates per clone). d Quantification of the percentage of cells in G1, S, and G2/M phases based on BrdU/PI FACS analysis (unpaired t-test; n = 2–3 biological replicates with 2 TKO and 2 QKO clones), performed 4 days after low density plating. e Quantification of  the percentage of AnnexinVneg/PIneg cells (live cells) in TKO and QKO populations 4 days after low density plating (unpaired t-test; n = 2 biological replicates per clone). Error bars represent ± s.e.m. Data shown as the mean and standard error of the mean