Fig. 3
Elimination of CD19-positive myeloma cells by CD19 CAR-T. a Expression profiles for CD19 on myeloma cells from patient M012, M016, M019, M022 and a merge of these four patients (b) generated by dSTORM. Distribution plots showing the relative CD19 density obtained after staining with anti-CD19 antibody on myeloma cells before and after treatment with CD19 CAR-T or untransduced control T cells. Densities are provided as logarithmic numbers (natural logarithm, Ln) of molecules per µm2. Probability density functions (PDFs) were fitted with a one or two-component log-normal function that was dependent on the fit-accuracy calculated with an Anderson-Darling test (rejected at a p-value < 0.05). For each patient, the percentage of myeloma cells that had been determined to be CD19-positive by flow cytometry is provided for comparison (red segments). For better visualization, the function of the isotype control is included in the CD19 staining for the merge panels (dotted blue line). Data for all patients are shown in Table 1 and Supplementary Fig. 4
