Fig. 3

GFP-PR₂₈ heterozygous mice show motor deficits. a Representative images of male control and GFP-PR₂₈ heterozygous mice at 2 months of age in tail-suspension test. b Quantification of the clasping time of mice in (a) during 2 -min test. Mann–Whitney test, n = 8, 6 mice. c Body weight of male control and GFP-PR₂₈ heterozygous mice at 2, 4, 6, and 12 months old. Two-way ANOVA, Bonferroni post hoc test; n = 5, 7 mice. d Quantification of time keeping on the edges of cages of male control and GFP-PR₂₈ heterozygous mice at 6 months of age. Mann–Whitney test, n = 19, 18 mice. e The numbers of hind limb foot slips on the balance beam test of male control and GFP-PR₂₈ heterozygous mice at 6 months of age. Mann–Whitney test, n = 19, 18 mice. f Representative images of male control and GFP-PR₂₈ heterozygous mice at 12 months of age in footprint test. Fore paws (red), hind paws (blue). Black squares indicate localization of hind paws. g Back stride length (left) of 6-, 12-month-old male control and GFP-PR₂₈ heterozygous mice measured in footprint test. Two-way ANOVA, Bonferroni post hoc test; 6 months, n = 22, 14 mice; 12 months, n = 16, 8 mice. h Back stride length (right) of 6-, 12-month-old male control and GFP-PR₂₈ heterozygous mice measured in footprint test. Two-way ANOVA, Bonferroni post hoc test; 6 months, n = 22, 14 mice; 12 months, n = 16, 8 mice. i Back-front distance measured in footprint test of male control and GFP-PR₂₈ heterozygous mice at 6, 12 months of age. Two-way ANOVA, Bonferroni post hoc test; 6 months, n = 22, 14 mice; 12 months, n = 16, 8 mice. All data are displayed as mean ± s.e.m. *P < 0.05, ***P < 0.001, ****P < 0.0001, n.s. not significant