Fig. 3
From: Structural basis of Q-dependent transcription antitermination
21Q-QBE interactions that mediate 21Q engagement. a QI and QII contact QBE from different faces of the DNA helix. Cyan, QI; green, QII; salmon, nontemplate strand; red, template strand. b Interactions between QII and QBE subsite 2. Salmon sticks, nontemplate strand; red sticks, template strand; black dashed lines, H-bonds (≤3.5 Å); red dashed line, salt bridge (≤4.5 Å). c Effects on 21Q-QBE binding affinity of substitutions of H5, H6, and L3 (mean ± SEM; 3 determinations). d Effects on antitermination of substitutions of H5, H6, and L3 (mean ± SEM; 3 determinations). e Effects on antitermination of substitutions in QBE subsite 1 (position −21) and subsite 2 (position −13), respectively (mean ± SEM; 3 determinations). Error bars represent mean ± SEM out of n = 3 experiments. Source data are provided as a Source Data file
