Fig. 5
From: A molecular switch from STAT2-IRF9 to ISGF3 underlies interferon-induced gene transcription
Complex formation of ISGF3 subunits and proximity labeling of interactors. a Raw 264.7 cells were treated for 1.5 h with IFN-β. Cell lysates were incubated with a biotinylated Oas1a-ISRE oligo, a biotinylated Isg15-ISRE oligo, or plasmid DNA. DNA-bound protein complexes were isolated by streptavidin affinity purification, followed by western blot analysis. b STAT1, STAT2, and IRF9 interactome dynamics in response to interferon treatment. Hierarchical cluster analysis of proteins significantly enriched upon treatment with IFN-β or IFN-γ. Proteins were filtered, which were at least twofold enriched above background (myc-BirA* or BirA*-NLS controls) in at least one condition at an adjusted p-value of < 0.01, and which showed at least a twofold increase in intensity after interferon induction when compared with steady-state conditions. For this filtered set of proteins, we computed the mean log2 LFQ protein ratio of the interferon-induced (2 and 18 h) and the steady-state condition and used these values to generate a hierarchical cluster analysis and heat maps in Perseus with default settings. Interactor names shown in red were found associated with more than one ISGF3 subunit across all experimental conditions, including interactors under resting conditions shown in Supplementary Data 7. Source data are provided as a source data file
