Fig. 4
Nanopore sequencing analysis for 1.5 MB Apollo file. Two MinION flowcells generated 267,152 1D2 reads of the 24-fragment Gibson Assembly of the Apollo file. a Base pair size of sequencing reads matches closely with the assembly size of 4590 bp. b We aligned each reference payload sequence to the sequencing reads. Each sequencing read resulting in an average of 17.99 alignments to different payloads. Ideally, each read should have 24 alignments. c We found an average sequencing coverage of 43x. Without any assembly, the same number of reads would have resulted in an average sequencing coverage of 2.6x. d We estimated raw sequencing quality by aggregating the average Phred quality score in each read. e Based on the reads that aligned to payloads, we calculated the average percent error for each base for insertions, deletion, and substitutions. f Substitution comparison across different bases revealed strong bias in between purines and pyrimidines
