Fig. 8
Triple-KD of three JmjC-demethylases reduces H3K9me3/2 demethylation and inhibits B-cell proliferation. shRNA lentiviruses targeting KDM2B, KDM3B, and KDM4C were mixed and used to infect splenic B cells. a The expression of cyclin E1, cyclin D2, Bcl-xL, and the three demethylases was analyzed by qRT-PCR 48 h after anti-IgM and LPS stimulation. b CFSE-labeled splenic B cells were infected with the shRNA lentivirus mixture, and cell proliferation was analyzed by flow cytometry at 72 h after anti-IgM and LPS stimulation. c Statistics for the percentage of B cells that underwent division following infection with the shRNA lentivirus mixture or control. d Changes in H3K9me2 at the cyclin E1 promoter were analyzed by ChIP-qPCR. All graphs with error bars indicate the mean ± SEM, *P < 0.05, **P < 0.01, Student’s t-test
