Fig. 1
From: Dissecting a heterotic gene through GradedPool-Seq mapping informs a rice-improvement strategy
The procedure of the GPS method. a F2 populations were generated by crossing two parent lines with distinct background and showed vastly different in plant height, followed by the self-pollination of F1 generation. Through the observation of plant height, F2 populations were classified from the highest to lowest bulk according to their frequency distribution. The letters M and F indicated the male parents and female parents, respectively. b The sequence reads of each bulk were aligned against the reference genome sequence to estimate allelic frequencies. We assumed that the depth at each SNP was 100×. If a variance was not related to the phenotype, it would present 50% reference reads and 50% alternate reads in all three bulks (right), whereas the related SNP would have great distinction of the ratio of reference reads to alternate reads among three bulks (left and middle). c Data analysis included Ridit analysis and reducing background noise approach. Several associated genetic intervals were mapped and indicated as red four-pointed star
