Fig. 2
From: Dissecting a heterotic gene through GradedPool-Seq mapping informs a rice-improvement strategy
QTL mapping and map-based cloning of OsMADS1GW3p6. a, b Identification of the genomic region consisting of OsMADS1GW3p6 via GPS approach. a The p value plot is the result of Ridit analysis for 1000-grain weight before noise-reduction algorithm. The -ln (p value) plot (Y axis) is plotted against SNP positions (X axis) on each of the 12 rice chromosomes. b After the strategy of reducing background noise, the results present as ratio plot. X-axis value is set at a midpoint at each defined genomic interval and Y-axis value corresponds to ratio. c The genotype of chromosome 3 of RIL-79. Black, white and gray bars represent the heterozygous genotype, and homozygous genotypes of GZ and FH respectively. d In the fine-scale mapping (lower bar) generated from the analysis of 1,079 segregating individuals, the QTL GW3p6 falls in the heterozygous interval. The numbers below the bar indicate the number of recombinants between GW3p6 and the molecular markers shown. e Genotyping of progeny homozygous for GW3p6 delimited the locus to a ~5.9 Kb genomic region between markers MP99 and MP100. f, h 1000-grain weight and grain length of recombinant F2 lines of RIL-79 (L1-L4). Data shown as means ± SD, n = 48. Source data are provided as a Source Data file. g Schematic diagram depicting the structure of OsMADS1 and OsMADS1GW3p6, red letters stand for the nucleotides of OsMADS1 non-homozygous segment. i The sequencing electrophoresis of non-homologous segment between OsMADS1 and OsMADS1GW3p6. j The schematic illustration of OsMADS1 functional domains, M represents the MADS domain, I represents the intervening domain, K represents the keratin-like domain, and C represents the C-terminal domain. Source data of Fig. 2f, h are provided in a Source Data file
