Fig. 6
From: PRKCSH contributes to tumorigenesis by selective boosting of IRE1 signaling pathway
Internal E/P domain of PRKCSH is required for boosting IRE1α activation under ER stress. a–c Immunoblot analysis of IRE1α phosphorylation (a), sXBP1 protein levels (b), and ERK phosphorylation (c) from L02 cells transfected with the control vector, WT PRKCSH, or its ΔE/P mutant; cells were treated with 10 μg/mL TM for the indicated time. d, e Immunoblot analysis of IRE1α phosphorylation (d) and sXBP1 protein levels (e) in L02 cells transfected with the control, ΔG2B, or ΔS/G2B mutant plasmids; cells were treated with 10 μg/mL TM for the indicated time. f, g Immunoblot analysis of IRE1α phosphorylation (f) and sXBP1 protein levels (g) in L02 cells transfected with the control, or E/P mutant plasmids; cells were treated with 10 μg/mL TM for the indicated time
