Fig. 4
Ectopic Foxg1 expression suppresses layer 4 fate. a Schematic diagram of in utero electroporation. Brains were introduced with pCAG:GFP and pCAG:empty (Control) or pCAG:GFP and pCAG:Foxg1 (Foxg1 GOF (CAG)) or pNeuroD1:Foxg1iresGFP (Foxg1 GOF (NeuroD1)). b–d′ Immunostaining (b–c′), reconstruction (d), and quantitative analysis of the morphology (d′) of GFP cells in P7 cortices. e Quantitative analysis of the distribution of GFP cells in P7 cortices. Cortical plate is divided into 10 BINs from the pia to the subplate. f–n′ Immunostaining of P7 cortices for GFP (green) with Rorβ (red) (f–h′), Brn2 (red) (i–k′), Satb2 (red) (l–n′), and Hoechst 33342 (blue). Dashed lines indicate layer 4 barrel structures. Closed arrowheads indicate GFP cells with Rorβ, Brn2, or Satb2 expression and open arrowheads indicate GFP cells without Rorβ, Brn2, or Satb2 expression. o Quantitative analysis of the percentage (±SEM) of GFP cells that express Rorβ or Brn2. p Schematic diagram of in utero electroporation. Brains were introduced with pCAG:GFP and pCAG:empty (Control) or pCAG:GFP and pCAG:Foxg1 (Foxg1 GOF). q Quantitative analysis of the distribution of GFP cells in P7 cortices. Cortical plate is divided into 10 BINs from the pia to the subplate. r–u Immunostaining of P7 cortices for GFP (green) with Rorβ (red) and Ctip2 (blue) staining (r, s) or Brn2 (red) and Satb2 (blue) staining (t, u). v Quantitative analysis of the percentage (±SEM) of GFP cells that express Rorβ, Brn2, Ctip2, or Satb2. * indicates P value <0.05 by Student’s t-test. Source data are provided as a Source Data file
