Fig. 5
Restoration of layer 4 fate upon removal of Foxg1 binding to COUP-TFI gene. a Schematic diagram of in utero electroporation. Brains were introduced with pCAG:GFP and pCAG:empty (Control) or pCAG:GFP and pCAG:Foxg1 (Foxg1 GOF). Combinations of gRNAs are as indicated. b–m Immunostaining of P7 cortices for GFP (green) with Rorβ (red) and Brn2 (blue) (b–e), or vGlut2 (red) and Cux1 (blue) (f–i), or Ctip2 (red) and Satb2 (blue) (j–m). Dashed lines indicate layer 4 barrel structures. Closed arrowheads indicate GFP cells with enriched vGlut2 signal, thalamocortical presynaptic terminals and open arrowheads indicate GFP cells without enriched vGlut2 signal (f–i). n–q Quantitative analysis of the percentage (±SEM) of GFP cells that express Rorβ (n), Brn2 (o), Satb2 (p), or Cux1 (q). * indicates P value <0.05 by Student’s t-test. Source data are provided as a Source Data file
