Fig. 6
Ectopic COUP-TFI expression promotes non-layer 4 cells to acquire layer 4 fate. a Schematic diagram of in utero electroporation. Brains were introduced with pCAG:GFP and pCAG:empty (Control) or pCAG:GFP and pCAG:COUP-TFI (COUP-TFI GOF). b Immunostaining of GFP (green), COUP-TFI (red), and Foxg1 (magenta) in E15.5 cortices. Closed arrowheads indicate GFP cells expressing both COUP-TFI and Foxg1. c Quantitative analysis of the distribution of GFP cells in P7 cortices. Cortical plate is divided into 10 BINs from the pia to the subplate. Quantitative analysis of the percentage (±SEM) of GFP cells with spiny stellate or pyramidal morphology. d–m″ Double immunohistochemistry of P8 cortices for GFP (green) with Ctip2/Zfpm2 (red) (d, e), Rorβ (red) (f, g), Cux1 (red) (h, i), Brn2 (red) (j, k), and vGlut2 (red) (l–m″). Dashed lines indicate layer 4 barrel structures. Closed arrowheads indicate ectopic vGlut2-positive thalamocortical presynaptic terminals as compared to the contralateral hemisphere. n Quantitative analysis of the percentage (±SEM) of GFP cells that express Rorβ, Cux1 or Brn2. o Schematic diagram of in utero electroporation. Brains were introduced with pCAG:GFP and pCAG:empty (Control) or pCAG:GFP and pCAG:COUP-TFI (COUP-TFI GOF). p Quantitative analysis of the distribution of GFP cells in P7 cortices. Cortical plate is divided into 10 BINs from the pia to the subplate. Quantitative analysis of the percentage (±SEM) of GFP cells with spiny stellate or pyramidal morphology. q–x Double immunohistochemistry for GFP (green) with Rorβ (red) (q, r), Brn2 (red) (s, t), Satb2 (red) (u, v), and vGlut2 (red) (w, x). y Quantitative analysis of the percentage (±SEM) of GFP cells that express Rorβ, Brn2 or Satb2. * indicates P value <0.05 by Student’s t-test. Source data are provided as a Source Data file
