Fig. 9
Removal of Egr target site derepresses Foxg1 and suppresses layer 4 fate. a Schematic diagram of gRNA design and in utero electroporation. b Quantitative analysis of the distribution of GFP cells in P7 cortices. Cortical plate is divided into 10 BINs from the pia to the subplate. c–k′ Immunohistochemistry of P7 cortices using GFP (green) and Rorβ (red) (c–e′), Satb2 (red) (f–h′), Ctip2 (red) (i–k′) antibodies and Hoechst 33342 (blue). l Quantitative analysis of the percentage (±SEM) of GFP cells with spiny stellate or pyramidal morphology and the percentage (±SEM) of GFP cells that express Rorβ or Satb2, or Ctip2. m Schematic diagram of gRNA design and in utero electroporation. Brains were introduced with pCAG:GFP and Cas9 only (Control) or pCAG:GFP and Cas9 with gRNAs (g68 + g352). n Immunostaining of E15.5 cortices for GFP (green) with Foxg1 (red) staining and Hoechst 33342 (blue). Dashed lines indicate the ventricular surface. VZ ventricular zone, IZ intermediate zone, CP cortical plate. o, o′ Quantitative analysis of the distribution of GFP cells in P7 cortices. Cortical plate is divided into 10 BINs from the pia to the subplate (o). Quantitative analysis of the percentage (±SEM) of GFP cells with spiny stellate or pyramidal morphology (o′). p–u′ Immunostaining of P7 cortices with GFP (green) and Rorβ (red) (p–q′), Satb2 (red) (r–s′), Ctip2 (red) (t–u′) antibodies and Hoechst 33342 (blue). v Quantitative analysis of the percentage (±SEM) of GFP cells that express Rorβ or Satb2. * indicates P value <0.05 by Student’s t-test. Source data are provided as a Source Data file
