Fig. 1
Overview of RAGE-Seq. Droplet-based scRNA-Seq is used to generate an initial barcoded cDNA library, which is split and simultaneously subjected to (i) short-read sequencing for 3’ expression profiling and (ii) targeted capture using custom probes followed by long-read sequencing. The short-read sequencing is used to generate highly accurate cell-barcode sequences which permit demultiplexing of the long-read data. Demultiplexed long-reads are subjected to de novo assembly and error correction to generate full-length BCR and TCR mRNA sequences, with single nucleotide accuracy. Transcriptome profiles generated from short-read sequencing can then be linked to the antigen–receptor sequence for each individual cell
