Fig. 1

LRR domains of the NLR family have a conserved multi-exon organization. a Scheme of domain and exon distribution in TLR4 and NLRP3. b Workflow for the selection of LRR exons used in c–f. c Length distribution of exons extracted in b. Box indicates 25th to 75th percentile, the middle line indicates the median, whiskers indicate min and max values. d All exons up to 200 bp in length plotted for their frequency distribution. Exons were divided in frame-shifting or frame-preserving. The typical length of LRRs (23 to 29 aa) is indicated as gray boxes. Colored boxes in the lower panel match assignment of groups in the following subfigures. e Genes in word clouds represent those genes whose exons contribute to the respective peaks in d. Word size is linearly dependent to the number of contributing exons. f Phylogenetic analysis of all genes contributing to the 4 major peaks in d. g Model of the NLRP3 LRR based on the human ribonuclease inhibitor LRR crystal structure. h LRR consensus sequences and structural alignments for the four groups identified in d–f. i Quantification of all LLR exons vs. non-LRR exons of all NLRs which would be frame-shifting if alternatively spliced. Independence of distribution was calculated using two-tailed Fischer’s exact test. See also Supplementary Fig. 1. Source data are provided as a Source Data file