Fig. 2
From: Structure-based mechanism for activation of the AAA+ GTPase McrB by the endonuclease McrC
Nucleotide binding at the McrBΔN interfaces. a–f Six panels, arranged in a clockwise cyclic manner, illustrating the interactions made with the nucleotide at the six interfaces. Green dotted lines represent potential hydrogen bonds (<3.6 Å) and ionic interactions (<4 Å). g Size exclusion chromatographic profile of McrB and its mutants in the presence of GTP using Superdex 200 column and in the presence of McrC and GTP using Superose 6 column (inset). h Comparison of GNP binding by McrB and its mutants. The binding curves of McrBR348A and McrBR349A overlapped. i Comparison of the GTPase activity of McrB and its mutants in the presence of McrC. j Nucleolytic activity of McrB and its mutants in the presence of McrC
