Fig. 5
DNA torsion sensitivity of different classes of chromatin binding proteins. a Plots of CHIP-seq fold enrichment versus TMP signal enrichment (expressed as the logarithm of the ratio of mean TMP-seq reads of a 400 bp genomic window centered on the binding sites and the background TMP-seq signals) of 8 different chromatin binding proteins (for details see methods). All error envelopes represent standard error of mean. b Violin plots showing the distribution of background corrected TMP-seq signals for the top 20% strongly represented CHIP-seq peaks for 10 different chromatin binding proteins. 1000 randomly selected data points are displayed on each violin. Red dots represent the median of the distribution. Fos, Jun and JunB, are transcription factors that bind known consensus sequence motifs in nucleosome-depleted regions. BRCA1 is a DNA damage repair protein. Ring1b is a component of the Polycomb group (PcG) proteins. BRD4 binds to acetylated histones in a DNA sequence-independent manner. Satb1, CTCF, Cohesin SA1, and SMC1 are chromatin architectural proteins. Satb1 CHIP-seq data, and TMP-seq data shown here can be accessed through GEO (Accession number: GSE123292). For all the other proteins, publicly available CHIP-seq data were used. See Methods for the accession numbers. Source data are provided as a Source Data file
