Fig. 8
Homeo domain is dispensable for high affinity binding but increases selection stringency. a Heatmap of ChIP-seq signals (read pileups) along a 4 kb window centered on binding peaks of different Satb1 domain constructs. Both N-C1 and ∆HD show higher signal enrichment at binding sites than FL Satb1. The dimerization domain N on its own shows no consistent signal enrichment. b Violin plots showing the distribution of the peak signal strengths for 3 different Satb1 constructs (Median peak strengths are shown as horizontal lines). c Venn diagram showing binding site overlap between all 3 domain constructs. d Venn diagram of FL versus N-C1 binding sites. N-C1 binds nearly all (~97%) of all FL binding sites and more than as many sites exclusively. e Heatmap of ChIP-seq signals (read pileups) (left column) and AT percentage (right column) of binding sites unique to FL Satb1 and N-C1. f, g Average plot of ChIP-seq signals (f) and AT percentage (g) along a 4 kb window across the binding sites that are exclusively bound by FL Satb1 or N-C1. h N-C1 and ∆HD bind cooperatively to sites that are common with FL Satb1. However, sites exclusively bound by N-C1 and N-C1-2 show no such cooperative pattern (inset). Error envelopes are plotted using standard error of the mean. i Distinct DNA-shape features help define FL Satb1 and N-C1 binding. Line plots showing average of MGW and ProT values across 40 bp windows centered on consensus sites bound by FL Satb1 and sites bound exclusively by N-C1 (n = 971) and ∆HD (n = 976) in nucleosome free regions. p-values in i are obtained using Mann–Whitney U test. Source data are provided as a Source Data file
