Fig. 5

p63 binding to epidermal enhancers precedes chromatin opening. a Alluvial plot showing the transition between 80% of epiboly and 24 hpf stages of the 22,583 peaks showing p63 binding (blue), chromatin accessibility (ATAC; red) or both (purple). Peaks showing no p63 binding in any of the two analyzed developmental stages were not considered. The transitions lead to eight groups of p63 BSs: group 1 (n = 332 peaks), group 2 (n = 145 peaks), group 3 (n = 43 peaks), group 4 (n = 1,284 peaks), group 5 (n = 3,619 peaks), group 6 (n = 1,227 peaks), group 7 (n = 896 peaks) and group 8 (n = 14,037). b Heatmaps of p63 and ATAC-seq signal in 80% of epiboly and 24 hpf stages for the groups resulting from the alluvial plot transitions in (a). Groups 2 and 3 were omitted due to their small number of peaks. c Heatmaps and average profiles of H3K4me3, H3K4me1 and H3K27ac in 80% of epiboly and 24 hpf for the group 4 of peaks from (a) (pioneered p63 BSs). d Motif enrichment analysis of the pioneered p63 BSs. The top-5 motifs are represented with their motif logos, position in the top-5, the TF name and the enrichment p-value in a color scale. e Top-10 enrichment of WT expression patterns for the genes corresponding to the pioneered p63 BSs. The -log₁₀ of p-value for each term is shown. f Genome tracks of p63 ChIP-seq, ATAC-seq, H3K27ac, H3K4me1, and H3K4me3 in the indicated developmental stages showing signal intensities in a region of chromosome 23. Pioneered p63 BSs associated to the epidermis-expressed gene lama5 are highlighted in light orange. The Genes track represents ENSEMBL annotated genes. See also Supplementary Fig. 4