Fig. 3

Inhibition of neuronal activity abolishes Rgs4 3′-UTR-dependent transport bias. a Scheme of experimental outline. Dot plots (b, d, f) and histograms (c, e, g) displaying percentage of anterograde moving MS2 only (b, c) or MS2+Rgs4 3′-UTR (d–g) reporter mRNA granules in rat hippocampal neurons, untreated, vehicle treated (DMSO) or silenced (100 µM CNQX, 50 µM AP5, 1 µM TTX) and after 1-h recovery. Data represent mean ± standard deviation of 3–4 independent experiments (individual experiments shown as gray dots). Asterisks represent p-values assessed by Tukey’s test post-hoc to one-way ANOVA analysis (*p < 0.05, **p < 0.01, ***p < 0.001). Data were obtained from 40-µm dendritic segments at a minimal distance of 20 µm from the cell body. At least 10 dendrites/condition/experiment were analyzed. Total number of dendrites (n_d) and tracks (n_t) analyzed per condition are indicated. Only displacements ≥1.5 µm were considered for analysis