Fig. 8
From: Glycine, serine and threonine metabolism confounds efficacy of complement-mediated killing
Effect of glyA deletion on glycine-cAMP/CRP-YhcD/NfrA/HtrE pathway. a, b Percent survival (a) and intracellular glycine (b) of E. coli Y3 and Y21 and their glyA-deleted mutants Y3△glyA and Y21△glyA, respectively. E. coli K12 and serum-resistant Y15 were used as negative and positive controls, respectively (n = 4). c–e ATP synthase activity (c), ATP level (d), and cAMP level (e) in the indicated strains (c, e, n = 3; d, n = 4). f crp expression in the absence of glyA (n = 4). g CRP expression in the absence of glyA. h qRT-PCR for expression of flhC, csgD, yhcD, nfrA, and htrE in the absence of glyA (n = 3). Results are displayed as mean ± SEM (a–f, g), and significant differences are identified (*p < 0.05, **p < 0.01) as determined by two-tailed Student’s t test (a–f, g)
