Fig. 5

Inflammation triggers transcriptomic changes in monocyte immediately upon entry into the small intestine. a Heat map demonstrating gene expression profiles of adoptively transferred CD11b⁺CD11c^lo/intCD64⁺ (MNP^CD64+) cells recovered small intestinal lamina propria (siLP) of non-manipulated and manipulated recipients at day 1, 2, and 5 after transfer. b Principal component analysis (PCA) representing transcriptomic profiles of donor BM monocytes before transfer and adoptively transferred MNP^CD64+ cells recovered from siLP of non-manipulated and manipulated recipients at day 1, 2, and 5 post-transfer. c–e Heat map showing hierarchal clustering of gene expression profiles of donor MNP^CD64+ cells recovered from the siLP at day 1 (c), day 2 (d), and day 5 (e) after transfer comparing non-manipulated and manipulated recipients. Transcriptomic analysis was performed by nanostring nCounter analysis and differentially expressed genes were determined by Qlucore Omics explorer with a statistical significance p-value of 0.05. Data were pooled from 2–3 independent experiments with a total of 2–5 mice as indicated in each section of the figure