Fig. 1 | Nature Communications

Fig. 1

From: Activation of neuronal genes via LINE-1 elements upon global DNA demethylation in human neural progenitors

Fig. 1

Disruption of DNMT1 in hNPCs leads to global loss of DNA methylation. a Schematic workflow of generation of DNMT1-KO hNPCs using LV.CRISPR-DNMT1. b Fluorescent immunostaining of 5mC 10 days post transduction with LV.CRISPR-DNMT1 and the control vector, scalebar 70 μm. c Control and DNMT1-KO hNPCs were allowed to incorporate BrdU between days 10–15 post transduction. Fluorescent immunostaining of 5mC and BrdU revealed that DNMT1-KO cells continued to divide and incorporate BrdU even after loss of DNA methylation, scalebar 25 μm. d The distribution of genome-wide mCpG/CpG ratios of 1 kb tiles is shown by Violin plots. Each data point is the mean mCpG/CpG ratio within each 1 kb tile. The black dot shows the mean of all 1 kb bin means. e UCSC screenshot of a part of chromosome 4 shows global loss of CpG methylation upon loss of DNMT1. f Violin plots show distribution of mCpG/CpG within all LINE, SINE, LTR, and SVA elements in hg38. The black dot shows the mean of all elements

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