Fig. 1
High-fiber diet protects mice against RSV-induced disease. a Female BALB/c mice were fed with control fiber (CF) or high-fiber (HF) diets for four weeks before and during RSV infection. Analyses were performed on day five post infection. b Percentage of weight loss post infection relative to initial body weight (day 0) (n = 9). c RSV viral load detected in lung tissue by real-time PCR (viral copies/g of lung tissue) (n = 9). d Total cell number and differential cell counts in bronchoalveolar lavage fluid (BALF) (n = 9). e Representative images of lung tissue section stained with hematoxylin and eosin (H&E) and its respective inflammation scores (n = 3). Scale bars = 100 μm. f Percentage of CD11c+CD86+ cells in axillary lymph nodes (n = 6). g, h Female BALB/c mice were fed with control fiber (CF), high-fiber (HF) or low-fiber (LF) diet for four weeks before and during RSV infection. Analyses were performed at day five post infection. g Percentage of weight loss post infection relative to initial body weight (day 0). h Total cell number in BALF (n = 5). i Analysis of the fecal microbiota composition from mice fed with CF and HF diets at the family level (relative abundance). j SCFA quantification in colonic luminal content (mg/g feces) (n = 8). k Spearman linear correlation between viral load and acetate quantification of animals fed with control and high-fiber diet. Data in a–e and j are from two independent experiments. All data are expressed as mean ± SEM and were compared using Kruskal–Wallis. In the other cases, Mann–Whitney was used. *p < 0.05, **p < 0.01, ***p < 0.001. Data in b, c, d, g, h, j are provided as a Source Data file
