Fig. 9
GPR43 is necessary for acetate protection of RSV-induced disease. a–g Gpr43 (G-protein coupled receptor 43) knockout mice (background in C57BL/6) and their controls (WT) were infected with RSV and treated with 200 mM acetate in drinking water. Analyses were performed on day five post infection. a Percentage weight loss post infection relative to original weight (day 0) (WT, n = 6; Gpr43−/−, n = 9). b RSV viral load detected in lung tissue by real-time PCR (viral copies/g of lung tissue) (WT, n = 6; Gpr43−/−, n = 9). c Total cell number and differential cell counting in BALF(WT, n = 6; Gpr43−/−, n = 8). d Immunohistochemistry staining of RSV in lung tissue sections. e mRNA expression of Ifnb1 in the lung (2-ΔCt analysis) (WT, n = 6; Gpr43−/−, n = 8). f mRNA expression of Oas1 and Isg15 genes in the lung (2-ΔCt analysis) (WT, n = 6; Gpr43−/−, n = 8). g IFN-β protein levels detected in the BALF or lung homogenate (WT, n = 4; Gpr43−/−, n = 6). h, i Pulmonary epithelial cells from female C57BL/6 wild-type and Gpr43−/− mice were treated with 260 µM acetate for 24 h and then infected with RSV (104 PFU/mL) for 24 h or 4 days. h cell viability evaluated by MTT assay. Control untreated/uninfected is showed as the dotted line. i IFN-β levels detected in supernatant of primary mouse lung cells culture (n = quadruplicate mean of 4 animals). j, k, Female C57BL6 wild-type and Gpr43−/− mice were infected with RSV (107 PFU/ml) for 24 h and then treated with acetate through the intranasal route (10 mM). After 24 h the lung was collected and processed for detection of IFNβ production by ELISA. All data are expressed as mean ± SEM. Data in a–g are from two independent experiments. Statistical significance between the groups was determined with Kruskal–Wallis. *p < 0.05, **p < 0.01, ***p < 0.001. Data in a, b, c, f, g, i are provided as a Source Data file
