Fig. 2 | Nature Communications

Fig. 2

From: Cytomembrane nanovaccines show therapeutic effects by mimicking tumor cells and antigen presenting cells

Fig. 2

Characterization of FM and MOF@FM. a Fusion of DCs and 4T1 cells by CLSM observation over red fluorescence of anti-CD44-APC antibody marked 4T1 and green fluorescence of anti-MHC II-FITC antibody labeled DCs, and the double-labeled FCs. Scale bar = 10 μm. b Flow cytometric analyses of anti-CD44-APC antibody marked 4T1 cells, anti-MHC II-FITC antibody labeled DCs and the double-labeled FCs. c SDS-PAGE protein analyses of CM, DM, and FM. d Western blotting analysis of membrane-specific and intracellular protein markers in the FCs lysate and FM. e SEM image of MOF. Scale bar = 200 nm. f TEM images of MOF@FM. Scale bar = 100 nm. g Zeta potentials of MOF, MOF@CM, MOF@DM and MOF@FM. Measurements were taken from distinct samples (n = 3). Source data are provided as a Source Data file

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