Fig. 5
circTP63 facilitates cell proliferation by relieving repression of miR-873-3p for FOXM1 expression. a A schematic model shows the putative binding sites of 22 predicted miRNAs on circTP63. b Luciferase activity of circTP63 in HEK293T cells transfected with miRNA mimics which are putative binding to the circTP63 sequence. Luciferase activity was normalized by Renila luciferase activity. c RIP was performed using AGO2 antibody in H2170 cells transfected with miR-873-3p mimics or mimics NC, then the enrichment of circTP63 was detected. d Luciferase reporter activity of circTP63 (left) and FOXM1-3′UTR (right) in HEK-293T cells co-transfected with miR-873-3p mimics or mimics NC. e Luciferase reporter activity of FOXM1–3′UTR in H2170 cells with circTP63 knockdown or overexpression. f Upper panel: circTP63 and FOXM1 were pulled down and enriched with 3′-end biotinylated miR-873-3p. Lower panel: binding activities of circTP63 and FOXM1 to 3’-end biotinylated miR-873-3p in H2170 cells with circTP63 overexpression. g FOXM1 expression in H226 and H2170 cells transfected with miR-873-3p mimics alone or co-transfected with circTP63. h Cell proliferation analysis for H226 and H2170 cells transfected with miR-873-3p mimics alone or co-transfected with circTP63. The error bars b–h represent s.d. (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001, two-tailed t-test. Source data are provided as a Source Data file
