Fig. 8
Mitophagy plays an essential role in HHV-8 productive replication. a RT-qPCR analysis of the copy number of encapsidated viral genome present in the culture media of control (sh-Luc) and vIRF-1- and/or NIX-depleted iBCBL-1 cells that were left untreated or treated with Dox for 4 days. Mean ± SD, n = 3. The p-value was determined by matched pair t-test (***p < 0.001). b Immunoblots of total-cell extracts derived from the same cultures as above (a) using antibodies to lytic antigens ORF45 and K8.1. The relative band intensities (RI) normalized to the loading control β-Actin are displayed beneath the corresponding panel. c RT-qPCR analysis of the mRNA expression of ORF45 and K8.1 in control (sh-Luc) and vIRF-1- and/or NIX-depleted iBCBL-1 cells that were left untreated or treated with Dox for 4 days. The relative expression levels (REL) normalized to 18S rRNA was calculated by dividing Dox sample by no-Dox control in each cell line. Data represent the mean ± SD of four independent experiments (n = 4). ns, not significant. d–f RT-qPCR analyses of encapsidated viral genome copy numbers in the media of iBCBL-1 cultures left untreated or treated with Dox for 2 days together with 10 µM TAT or TAT-PD1 peptides (d), 20 µM liensinine or vehicle (DMSO) (e), and Mdivi-1 (f) at the indicated concentrations. Mean ± SD, n = 3. The p-value was determined by matched pair t-test (*p < 0.05, **p < 0.01, and ***p < 0.001). Source data are provided as a Source Data file
