Fig. 9
Clinical variables relevant in transfusion-associated necrotizing enterocolitis (NEC). Scatter plots show severity of NEC-like injury (means ± standard error) following: a red blood cell (RBC) transfusions in mice with severe (hematocrit 20–24%) vs. moderate anemia (hematocrit 25–30%); b transfusions administered soon after the hematocrit dropped to 20–24% (on P10) vs. 24 h later (P11); c transfusions with leukoreduced, packed RBCs vs. RBCs that were leukoreduced, washed, and resuspended in phosphate-buffered saline (PBS) before storage; d transfusions with RBCs stored for 7 vs. 14 days; e single vs. multiple RBC transfusions. N = 18 mice per group in all above experiments; Mann–Whitney U test, **P < 0.01, †P < 0.001. f Scatter plot shows positive correlation (Spearman’s r = 0.865) between number of transfusions and severity of injury; †P < 0.001. All error bars show standard error of the mean
