Fig. 3
Genetic screening of the function of candidate N NLR immune receptor interacting proteins in N-mediated resistance to TMV. a Phenotypic observation of the TMV-U1-incoluted N-containing transgenic N. benthamiana plants after being silenced with various target genes as indicted. Various recombinant TRV vectors containing different gene fragments were inoculated onto the N-containing transgenic N. benthamiana plants. 10 days later, TMV-U1 was rub-inoculated onto the upper leaves. Photographs were taken at 7 days after TMV inoculation and representative results are shown. These silencing experiments were repeated 2–3 times. b Analysis of the TMV RNA corresponding to the movement protein coding region in the upper uninoculated leaves by RT-PCR (TMV-MP, top panel). eIF4A was used as an internal control to validate the equal amount of total RNA used for RT-PCR (bottom panel). c Silencing of NbUBR7 enhances N-mediated resistance to TMV. TMV-U1-GFP was rub-inoculated onto vector control or the NbUBR7-silenced N-containing transgenic N. benthamiana leaves. Photographs were taken under UV light at 5 days post inoculation and representative results are shown (upper panels). d The expression level of GFP in the inoculated leaves was examined by western blot analysis using antibodies against GFP (top panel). PEPC served as loading control for equal protein loading (bottom panel). The molecular weight size markers in kDa are indicated at the left of each panel. Source data are provided as a Source Data file
