Fig. 6

RRM2 is deacetylated following DNA damage in an ATR-dependent manner. a H1299 cells were exposed to IR (10 Gy) or treated with camptothecin (CPT, 2 µM) for 6 h, followed by IP with RRM2 antibody, and analysis of RRM2 acetylation. b H1299 cells expressing exogenous Flag-Sirt2 were treated with IR or CPT, followed by co-IP using Flag antibody. Flag-Sirt2-associated RRM2 was analyzed by western blot using RRM2 antibody. c, d H1299 cells were transfected with ATM siRNA, ATR siRNA, or DNA-PKcs siRNA or treated with ATM inhibitor (KU55933, 10 µM), ATR inhibitor (VE821, 2 µM), or DNA-PK inhibitor (NU7441, 10 µM), followed by IR, RRM2 IP, and analysis of RRM2 acetylation