Fig. 8
From: Acetylation regulates ribonucleotide reductase activity and cancer cell growth
RRM2 acetylation at K95 results in reduction of dNTP pool size and growth inhibition. a After endogenous RRM2 was depleted from H1299 cells using 3′-UTR-targeted RRM2 shRNA, Flag-tagged WT or individual acetyl-mimetic RRM2 mutant(s) were exogenously expressed in RRM2-deficient H1299 cells. b–h Intracellular dNTPs, DNA replication fork progression, cell proliferation, and cell growth were analyzed by HPLC, DNA fiber assay, BrdU/7-AAD proliferation assay, and colony-formation assay, respectively, in RRM2-deficient H1299 cells expressing empty vector (EV), Flag-tagged WT, or individual acetyl-mimetic RRM2 mutant(s). The error bars indicate ± s.d. of three separate experiments. *P <0.05, **P <0.01, ***P <0.001, by two-tailed t test
