Fig. 7
From: Two-step chromosome segregation in the stalked budding bacterium Hyphomonas neptunium
Spatial arrangement of the H. neptunium chromosome. a Schematic showing the locations of chromosomal loci tagged with the Y. pestis ParB-parSpMT1 system. b Subcellular localization of ten different chromosomal loci distributed evenly across the H. neptunium chromosome. Strains (parB-yfp PZn::PZn-mCherry-parBpMT1) carrying parSpMT1 at the indicated chromosomal locations (AJ64-69, SRE13-15, AJ49) were grown in MB medium and analyzed by DIC and fluorescence microscopy. The relative subcellular positions of the tagged loci (as reflected by the centers of mass of the respective foci) were determined in swarmer and early stalked G1-phase cells and plotted versus the total cell length, with 0% corresponding to the old (flagellated) pole and 100% to the new (future stalked) pole. n = 75 cells (5°), 118 cells (53°), 60 cells (85°), 48 cells (129°), 53 cells (172°), 50 cells (186°), 67 cells (230°), 64 cells (272°), 50 cells (310°), and 52 cells (357°)
