Fig. 1
From: RIF1 promotes replication fork protection and efficient restart to maintain genome stability
RIF1 is recruited to the stalled replication forks. a Schematic representation of iPOND experiment. b Volcano plot showing the results for average fold-change to identify significantly upregulated proteins upon HU treatment based on H:L ratio in the SILAC experiment. The x-axis ('2Log Difference HU/NT) represents the fold upregulation. Data points in blue represent proteins that are upregulated >2-fold; RIF1 is indicated in red. c Bar graph showing fold upregulation of a selection of proteins upon HU treatment based on their SILAC H:L ratios (error bars represent standard deviation). d Representative micrographs showing co-localization of RIF1 (green) to sites of DNA replication as marked by EdU (red) in the presence or absence of HU in WT and 53bp1−/− cells. Nucleus was stained with DAPI (blue). e Quantitation of d showing the percentage of cells, which show co-localization between EdU and RIF1(error bars represent standard deviation)
