Fig. 2

RLRs dictate the transcriptome response to WNV infection. a Experimental design. BMMs were infected with WNV at MOI 2.5 for 24 or 48 h when RNA and supernatants were harvested for analysis. RNA was submitted to RNA sequencing and analyzed through an Immuno-informatics pipeline. Gene expression was determined by comparing the number of reads in the infected condition compared to that genotype’s mock condition. b Bar graph representing the total number of differentially expressed genes by genotype and time point. Induced genes are shown in red as positive numbers while suppressed genes are shown in blue as negative numbers. A single value is shown for each genotype and time point. c) Heatmap of global gene expression. Genes significantly changed in the WT response compared to the WT mock are shown on the heatmap as horizontal rows. The columns are each genotype at the noted time points. Genes that had more mapped reads in the infected condition are shown in red while genes that had fewer mapped reads in the infected condition are shown in blue. On the right, genes with similar expression are grouped into blocks and assigned functional terms. n = 3 independent infections, RNA preparations, and sequencing results. Source data are provided as a Source Data file