Fig. 5

Unique RIG-I, MDA5, and LGP2 transcriptional responses following WNV infection. a Venn diagram showing the overlap in numbers of RLR-dependent. Red numbers are induced genes while blue numbers are suppressed genes. b Heatmap showing the significantly enriched induced (top, in red) and suppressed (bottom, in blue) GO terms across RIG-I, MDA5, and LGP2 deficient cells. Grey indicates that GO term was not significantly enriched in the samples and are therefore dependent on the specific RLRs for gene module regulation. Each horizontal line represents a GO term while the columns are the genotype of the samples. The color represents the adjusted p-value (co-expression) of the enrichment where a darker color indicates a smaller (more significant) p-value. c Radar plots of the RIG-I, MDA5 and LGP2 deficient responses. Genes were categorized, and proportions calculated as in Fig. 4. Top radar plots are the categorization of the genes that were present in each genotype’s response. Bottom radar plots are the categorization for the RLR-dependent genes (genes altered in WT response, but unaltered in RLR-KO response). RIG-I is shown in yellow, MDA5 is shown in red, and LGP2 is shown in blue. n = 3 (DKO), 4 (LGP2^−/−, MDA5^−/−, RIG-I WT, RIG-I^−/−), or 5 (WT B6) independent infections, RNA preparations, and sequencing results. Source data are provided as a Source Data file