Fig. 7

ATF4 and SMAD4 are potential mediators of the RLR-based macrophage response to WNV. a Upstream regulator analysis predicted regulators (Z-scores) are shown by heatmap where red shows predicted activation and blue shows predicted inhibition. This analysis was filtered to remove all regulators that had the same directionality in WT and DKO data sets as well as any regulator that had a zero Z-score in either dataset (bottom). b, c Network analysis of ATF4 target genes (b), SMAD4 target genes (c) and M1/M2 signature genes (both) in the WT response (left) and the DKO response (right). Predicted upstream transcriptional regulators are noted as orange octagons with the remaining nodes in our network are labeled by gene name. In the networks, shapes indicate gene category (circles—transcription factor target gene, octagon—regulator of interest, diamonds—M1 genes, triangles—M2 genes, and squares—activators). The colors are the directionality of the gene in our dataset (red is induced, blue is suppressed, and white is not changed compared to mock). n = 3 independent infections, RNA preparations, and sequencing results. Source data are provided as a Source Data file