Fig. 3

Changes in lipoprotein size distribution revealed through coupling Native-polyacrylamide gel electrophoresis (Native-PAGE) to LipoGlo. a Representative image of the fluorescent DiI-LDL migration standard and LipoGlo emission from wild-type (WT), mtp^−/−, and apoC2^−/− genotypes (4 days post fertilization (dpf)). ApoB-LPs are divided into four classes based on their mobility, including ZM (zero mobility) and three classes of serum ApoB-LPs (very-low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), LDL). Image is a composite of chemiluminescent (LipoGlo, blue) and fluorescent (DiI-LDL, orange) exposures. Gel is a representative image from one of the three independent experiments performed. b Vertical plot profile of gel image displayed in a, note that the ZM peak has been appended to highlight differences in serum lipoprotein classes. c–e Representative LipoGlo PAGE gels (one of three independent experiments shown) and f–h quantification of pooled LipoGlo PAGE gel data from larval lysates used in Fig. 2. Note that relative abundance was quantified, so the sum of all species will always equal 100% despite changes in total abundance over time. Relative abundance of subclasses is color coded as shown in a. Upward-facing arrowheads (green) indicate significant enrichment of that species at that time point compared to WT, and downward-facing arrowheads (magenta) indicate depletion using the Games–Howell test with a threshold of p < 0.05. f Subclass abundance at each day of larval development in WT (degrees of freedom (DF) = 5, n = 9, Welch’s analysis of variance (ANOVA) p < 0.0001 for each subclass over time), mtp^−/− (DF = 11, n = 9, two-way robust ANOVA p < 0.001 for VLDL and LDL, Games–Howell p < 0.01), and apoC2^−/− (DF = 11, n = 9, two-way robust ANOVA p < 0.01 for all classes, Games–Howell p < .005) genetic backgrounds. g Subclass abundance from 3 to 5 dpf in larvae treated with 10 μM lomitapide or vehicle control (DF = 11, n = 9, two-way robust ANOVA p < 0.001 for all classes except IDL, Games–Howell p < 0.01). h Subclass abundance from 10 to 15 dpf in larvae subjected to a fasting and re-feeding paradigm. The first bracket delineates changes relative to time 0 (the onset of the fasting period), and the second bracket delineates changes relative to time point 48 (the onset of the re-feeding period) (DF = 10, n = 9, Welch’s ANOVA p < 0.0001 for each subclass over time, Games–Howell p < 0.01). Supplementary Fig. 4 displays standard deviations for f–h. Results represent pooled data from three independent experiments, “n” denotes number of samples per data point. Source data are provided as a Source Data file