Fig. 4
From: Augmentation of myocardial If dysregulates calcium homeostasis and causes adverse cardiac remodeling
Transcriptional changes and expression of Ca2+ handling proteins in HCN4tg/wt hearts. qPCR and Immunoblots from heart samples of 6 months-old mice. qPCR measurements of gene transcription related to a cell growth, b the fetal gene program and hypertrophy, c Na+/Ca2+-handling, d murine HCN channels, and e cardiac ion channels (6 months-old mice n = 4–8 animals/group). The bars show the transgenic gene expression normalized to wild type expression level = 1.0, which is indicated by the dashed line. *P < 0.05; **P < 0.01; ***P < 0.001 (unpaired t-test). f Representative immunoblots of Ca2+-handling proteins. g Protein expression of transgenic animals compared to wildtype littermates obtained from immunoblots (6 months-old mice n = 3 animals/group). Two replicate immunoblots per animal, in pPln-S16 and pPln-T17 one replicate immunoblot per animal. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control. *P < 0.05; **P < 0.01 (unpaired t-test). Data are shown as mean ± s.e.m. Source data are provided as a Source data file
