Fig. 8
From: Augmentation of myocardial If dysregulates calcium homeostasis and causes adverse cardiac remodeling
Electrophysiological properties of HCN4tg/wt cardiomyocytes. a Representative action potential (AP) traces of wild type (gray) and HCN4tg/wt cardiomyocytes (black). b Quantitative data on AP amplitude of wild type (gray) and HCN4tg/wt (black) cardiomyocytes at different pacing rates (0.5, 1, 2 Hz) showed decreased amplitude of overshoot in cells from transgenic mice. AP amplitudes of HCN4tg/wt cardiomyocytes decreased significantly with higher pacing rates. c HCN4tg/wt cardiomyocytes show depolarized RMP. d–f AP properties were evaluated at different pacing rates (0.5, 1, 2 Hz). AP duration at 20 (APD20), 50 (APD50), and 90% (APD90) repolarization are depicted. Data are presented as boxplots. Box limits delineate interquartile values, centerlines represent the median and whiskers indicate the range (wild type: n = 20 cells, HCN4tg/wt: n = 17 cells, for RMP: wild type: n = 14 cells, HCN4tg/wt: n = 12 cells derived from n = 5 animals/group; 3 months of age; *P < 0.05, **P < 0.01, ***P < 0.001; unpaired t-test; for evaluation of rate-dependency of AP amplitudes: #P < 0.05; nonparametric ANOVA followed by Dunn’s test). Source data are provided as a Source data file
