Fig. 2
Myo6 binding to a lipid bilayer is curvature sensitive. a Super-resolution microscopy (SRM) of myo6 flowers growing over 60 min (150 nM myo6, 10% green fluorescent protein (GFP)-myo6; bilayer unlabelled). The colour-coded time stamps were obtained from SRM images integrated over 1 min time periods (14–15 and 44-45 min, respectively), bilayer unlabelled. Arrow heads mark hotspots of GFP-myo6 binding. The SRM image integrated over 60 min shows the new appearance and growth of the myo6 flowers with the colour encoding the progress in time. Zoomed-in image shows freshly bound myo6 (red) at hotspots (arrows) where the membrane border bulges outward, away from the pore centre; scale bars 1 µm; scale bar in inset (*) 200 nm. b GFP-myo6 detected during three colour-coded, 1 min time intervals superimposed onto the respective perimeter of the flowers (solid lines, ‘Methods’). Hotspots of GFP-myo6 marked by arrow heads; scale bar 1000 nm. c Perimeter determined by integrating SRM data over time t; n = 253, 561 and 948 measurements in total for 50 (black filled circles), 150 (grey filled circles) and 300 nM myo6 (blue unfilled circles) from 20 to 70 flowers in each condition. Same data shown as scatter plot (area vs perimeter) and as mean perimeter (mean ± s.d.) sorted by time t. Green line, theoretical model (see below). Source data are provided as a Source Data file
