Fig. 5
Biodistribution and antitumor effect in CT-26 tumor model. a Real-time in vivo fluorescent imaging in mice after they were intravenously injected with ICG-HAuNS (indocyanine green (ICG): 4 mg/kg; hollow gold nanospheres (HAuNS): 8 mg/kg), FAL-ICG-HAuNS (ICG: 4 mg/kg; HAuNS: 8 mg/kg), DiD@Hb-lipo (Hb: 20 mg/kg), or DiD@FAL-Hb-lipo (Hb: 20 mg/kg). Ex: 649 nm for DiD; 735 nm for ICG. b CT-26 tumor growth curves after treatment with saline, ICG-HAuNS (ICG: 0.5 mg/kg and HAuNS: 1 mg/kg per injection), FAL-ICG-HAuNS (ICG: 0.5 mg/kg and HAuNS: 1 mg/kg per injection), ICG-HAuNS (ICG: 0.5 mg/kg and HAuNS: 1 mg/kg per injection) plus Hb-lipo group (20 mg/kg per injection), or FAL-ICG-HAuNS (ICG: 0.5 mg/kg and HAuNS: 1 mg/kg per injection) plus FAL-Hb-lipo (20 mg/kg per injection). Laser power: 1 W/cm2, 2 min, n = 6. c Kaplan–Meier plots on animal survival, n = 6. d Representative Ki-67 and hematoxylin and eosin staining results in each group. Scale bars, 300 μm. All data were analyzed with one-way analysis of variance test. All error bars are expressed as ±SD
