Fig. 5

Adipose ILC1s induce adipose fibrosis through IFN-γ production. C57BL/6 and Ifng^−/− mice were fed a HFD for 2 weeks, and 5 × 10⁴ adipose ILC1s were sorted (>95% purity) from adipose tissue of each group and adoptively transferred i.v. into separate Prkdc^−/−IL2rg^−/− mice. The recipient mice were fed a HFD for 6 weeks. Three weeks after HFD, adipose ILC1s were adoptively transferred into recipients again. a Schematic of the experiment. b Body weight. c Fasting blood glucose. d Glucose tolerance test. e Expression levels of fibrosis-related genes, assessed by q-PCR. f Representative Masson’s trichrome C and Sirius red staining in VAT of mice with different treatment (scale bar: 100 μm). Arrows indicate positive staining areas. ^*P < 0.05 vs. CHOW group; ^**P < 0.01 vs. CHOW group; ^#P < 0.05 vs. HFD+Ifng^−/− ILC1 group; ^##P < 0.01 vs. HFD+Ifng^−/− ILC1 group (two-way ANOVA tests followed by Bonferroni post hoc test). The data are representative of two independent experiments, with n = 4–6 mice per group. Error bars indicate s.d.